![]() Advantages of using a prestained protein ladder: Unstained protein ladders. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel). In this article: Prestained protein ladders. The unique Strep -tag affinity peptide allows detection and molecular weight determination on western blots. Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Precision Plus Protein Unstained Standards are a mixture of ten Strep-tagged, recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD).Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF) Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Compatible with chemiluminescent substrates and fluorescent secondary antibodies. The system includes plasmids which express 10. The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). Western blot confirmationdetect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein Applications Western blotting: detection of the two unstained bands via the detection method used for the target protein. We have created the Penn State Protein Ladder system to produce protein molecular weight markers easily and inexpensively (less than a penny a lane). Each protein can be stained with a different color for easy identification or labeled with affinity tags for western blot detection. Precision Plus Protein All Blue Standards are a mixture of ten blue-stained recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). Recombinant protein ladders are engineered to produce tight bands, evenly spaced molecular weights, and other specific traits. The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site. Protein ladders are used in experiments to estimate the size of proteins of interest and are resolved using gel electrophoresis. What should I do Here are some suggestions: Make sure that the correct amount of standard is loaded per lane.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |